商品名称：
Acanthamoeba healyi Moura et al.
isolation：
sewage outfall, Bethany Beach, Maryland, 1984
strain designations：
BB-12-3SW-MX
biosafety level：
2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
medium：
ATCC® Medium 711: PYB ATCC® Medium 711: PYB ATCC® Medium 997: Fresh water ameba medium
growth conditions：
Temperature: 25.0°CProtocol: ATCCNO: 50655 SPEC: The strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Remove the frozen ampule from the dry ice and transfer directly to a 35C water bath. After thawing the ampule, transfer the contents to the surface of an ATCC medium 997 or 711 agar plate (20 x 100 mm). Spread the material evenly over the surface of the plate with a sterile spread bar. The food bacterium, Enterobacter aerogenes ATCC 13048, is present in the thawed material. Wrap the plate with time tape or parafilm and incubate upright at 25C. Many trophozoites should be visible within 2-3 days. Transfer the culture every 28 days as follows: Aseptically remove a small block of agar (approximately 5 mm square) containing cysts and, at the edge of an ATCC medium 997 or 711 agar plate containing a lawn of Enterobacter aerogenes ATCC 13048, invert the block onto the surface. Wrap the new plate and incubate as above. The trophozoites will migrate away from the agar block to the opposite edge of the plate. Continue to subculture as above.
name of depositor：
TK Sawyer
